Fpn1 is increased in the duodenum of 12-month-old mice affected by β-thalassemia intermedia. Duodenum of (A) +/+-12M and (B) th3/+-12M mice. This assay confirmed our Q-PCR data that indicated up-regulation of Fpn1 in 1-year-old th3/+ mice compared to +/+ animals. (immunoperoxidase; original magnification, ×66). Images were captured on a Nikon Eclipse E800 microscope (Nikon, Melville, NY) with a Retiga Exi camera (Qimaging, Burnaby, BC, Canada) and a Plan Fluor 20x/0.75 NA objective, then acquired using IP Lab 3.65a software (Scanalytics, Fairfax, VA). Brightness/contrast and color balance were adjusted using Adobe Photoshop 7.0.1 (Adobe Systems, San Jose, CA).