Serum iron levels, percentage of Tf saturation, and LPI levels are elevated in mice affected by β-thalassemia major. Mice affected by β-thalassemia major showed a predominant iron deposition in hepatic parenchymal cells. To evaluate whether mice affected by β-thalassemia major had elevated levels of NTBI, we investigated mice at 2 months, given transplants or not so treated. These mice showed the same serum iron, percentage of Tf saturation, and LPI values. Therefore, to simplify our graphs, we combined the animals given transplants and not given transplants into a single group for each genotype. Error bars represent SD performed on at least 5 animals per group. *P < .05, **P < .01, and ***P < .001 relative to controls using the Dunnett multiple comparison test.