American Society of Hematology

Figure 1

Platelet particle formation, size, and annexin binding induced by anti–GPIIIa49-66 Ab or Ca2+ ionophore. (A) Studies with anti–GPIIIa49-66 Ab. Gel-filtered platelets were labeled with either CD61-FITC (left panels) or annexin-FITC (right panels) incubated with buffer (A,E), control IgG (B,F), or anti–GPIIIa49-66 (C,G) for 4 hours at 37°C and analyzed by flow cytometry (representative of 3 experiments). (B) Studies with Ca2+ ionophore. Similar studies were performed with Ca2+ ionophore as agonist (D,H). The numbers in the quadrants refer to the percentage of cells in the quadrant as determined by fluorescent labeling.

Platelet particle formation, size, and annexin binding induced by anti–GPIIIa49-66 Ab or Ca²⁺ ionophore. (A) Studies with anti–GPIIIa49-66 Ab. Gel-filtered platelets were labeled with either CD61-FITC (left panels) or annexin-FITC (right panels) incubated with buffer (A,E), control IgG (B,F), or anti–GPIIIa49-66 (C,G) for 4 hours at 37°C and analyzed by flow cytometry (representative of 3 experiments). (B) Studies with Ca²⁺ ionophore. Similar studies were performed with Ca²⁺ ionophore as agonist (D,H). The numbers in the quadrants refer to the percentage of cells in the quadrant as determined by fluorescent labeling.

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