Dynamic c-myb expression and migration of c-myb–positive cells from the ventral wall of DA to PBI. (A-D) WISH shows c-myb expression pattern at 30-hpf, 2-dpf, 3-dpf, and 5-dpf embryos. The inset is a high magnification of the boxed region. The arrow in panel D indicates c-myb expression in the kidney. (E-G) Lateral view of 30-hpf, 2-dpf, and 3-dpf embryo. Embryos were uncaged in the anterior part (blue cross in panel E) of ventral wall of DA, fixed at 2 dpf or 3 dpf, and co-stained with flu and c-myb RNA. (H, I) Confocal images of the boxed region 1 in panel F show flu and c-myb signals in the original uncaged region (corresponding to the region marked by the blue cross in panel E) at 2 dpf. (H/I) Merged view of panels H and I. (H'/I') Superimposed view of panels H/I and DIC image. (J, K) Confocal images of the boxed region 2 in panel F show flu and c-myb signals in the PBI at 2 dpf. (J/K) Merged image of panels J and K. (J'/K') Superimposed view of panels J/K and DIC image. (L, M) Confocal images of the boxed region 3 in panel G show flu and c-myb signals in the PBI at 3 dpf. (L/M) Merged image of panels L and M. (L'/M') Superimposed view of panels L/M and DIC image. Arrows (H-L'/M') indicate the co-staining of flu and c-myb.