Rh antigen and Rh complex expression levels of red cell subsets from patients apparently affected by either somatic recombination-associated RH duplication or deletion. Expression of (A) Rh antigens C, e, and c, and of (B) total Rh protein, RhAG, and LW of C⁺D⁺ and C⁻D⁻ RBC subpopulations (C⁺D⁺ subpop and C⁻D⁻ subpop) of CcDdee patients with either somatic recombination-associated RH duplication (duplication: patients 1, 2, and 6) or monoallelic RH deletion (resulting in RH hemizygosity; deletion: patient 3) is shown. For comparison, values of control CcDdee (n = 3) and ccddee (n = 3) RBC samples are depicted. Monoallelic RH deletion in patient 3 is reflected by markedly reduced e and c expression of his C⁻D⁻ subpopulation, compared with ccddee controls. In contrast, C⁻D⁻ subpopulations from patients with RH duplication express e and c levels similar to ccddee controls. Moreover, the partial RH hemizygosity of patient 3 was also associated with particularly low expression of total Rh protein, RhAG, and LW of his C⁻D⁻ subset. Data are shown as the averaged mean fluorescence intensities relative to values of CcDdee control RBCs set as 100%. Error bars represent SD.