Flow cytometric surface and cell-cycle analysis. A total of 12 representative mice with LPD were examined and their analysis presented in order in panels A-F. The mice are numbered from 1 to 12, and data from the same animal are shown for both spleen and peritoneal lavage cells (A-D). (A-B) Single-parameter DNA content histograms for spleen and peritoneal cells. (C-D) Two-parameter IgM versus DNA content (FL2-A vs FL1-H) for spleen and peritoneal cells (E-F) Expression of IgM versus CD5 (FL1-H vs FL2-H) and IgM versus B220 (FL1 vs FL3-H), respectively, for spleen cells. Examples of splenic hyperdiploidy can be seen in panel A, mice nos. 2, 4, 8, and 9. Further resolution of these aberrant subpopulations can be better appreciated in the corresponding panel C. The smaller aneuploid populations seen in mice nos. 11 and 12 can be better appreciated in the 2-color analysis shown in panel C.