Growth factors and coculture with BMSCs do not protect against enzastaurin-induced WM cell cytotoxicity. (A) BCWM.1 cells were cultured with DOPPA (200 nM) for 10 and 20 minutes. Whole-cell lysates were subjected to Western blotting using anti–p-PKCβ, anti-PKCβ, and α-tubulin antibodies. (B) BCWM.1 cells were cultured with control media in the absence and presence of DOPPA (200 nM) and treated with enzastaurin (2.5 to 10 μM) for 48 hours. Inhibition of proliferation was assessed by thymidine uptake assay. (C) BCWM.1 cells were cultured with control media, and with 5 μM, 7.5 μM, and 10 μM enzastaurin for 48 hours, in the presence or absence of BMSCs. Cell proliferation was assessed using [³H]-thymidine uptake assay. (D) BCWM.1 cells were cultured with control media in the absence and presence of IL-6 (25 ng/mL) and treated with enzastaurin (5 and 10 μM) for 48 hours. Inhibition of proliferation was assessed by thymidine uptake assay. All data represent mean (± SD) of triplicate experiment.