American Society of Hematology

Figure 4

$Figure 4. Northern and Western blot analyses of PCFT mutants. (A) Northern blot analysis. Total RNA (20 μg) was fractionated on a formaldehyde-agarose gel, and the blot was successively probed with a PCFT fragment and β-actin. The images represent 1 of 2 independent experiments. (B) Western blot analysis. An equal amount of lysate (20 μg) was loaded on SDS gels, and the blots were probed with a peptide antibody directed to the C-terminus of PCFT. The numbers on the left indicate the molecular sizes of the protein ladder. The left and right panels represent 2 different analyzes each with mock and wild-type PCFT as controls. Each blot is representative of 3 separate experiments.$

Northern and Western blot analyses of PCFT mutants. (A) Northern blot analysis. Total RNA (20 μg) was fractionated on a formaldehyde-agarose gel, and the blot was successively probed with a PCFT fragment and β-actin. The images represent 1 of 2 independent experiments. (B) Western blot analysis. An equal amount of lysate (20 μg) was loaded on SDS gels, and the blots were probed with a peptide antibody directed to the C-terminus of PCFT. The numbers on the left indicate the molecular sizes of the protein ladder. The left and right panels represent 2 different analyzes each with mock and wild-type PCFT as controls. Each blot is representative of 3 separate experiments.

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