Figure 6
![Figure 6. Structure of VB22B sc(Fv)2 minibody. A noncovalent (55 kDa) “diabody” (middle of figure) can be generated by taking the VH and VL regions of a whole anti-Mpl monoclonal IgG (left) and coupling them via a 5-amino acid linker (GGGGS). VB22B sc(Fv)2 is made by binding one VH and one VL fragment together with a 15-amino acid linker [(GGGGS)3] and then binding 2 of these constructs together with the same 15-amino acid linker (middle). Both the diabody and the VB22Bsc(Fv)2 bind and activate the TPO receptor (right). Illustration by Paulette Dennis.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/109/11/10.1182_blood-2006-10-019315/4/zh80110703760006.jpeg?Expires=1723206682&Signature=gf-HcutsbRBKNBdHzH2bKggPKSJaFkXC6rSfhk-mXv5h8APOAjX-R2gKR-d6ay6RqpI1gvlF4KFfD4miF~k1hVE4hg1vlSmTBjB7ztfqPClrgJJ5fIw-O7zDnxo8PxsCUMHqXAKCTq33HJj881Ug8M3zXCKALw6QBUqqp0jlKQSKG-kNk8yVoKmvjE66duScxepFf8Yr9Pl6~39QYF4CIGw0pcXzG2rBlPglUx-kuVMHIQcEaEKoiiyOJQKqd96F3VL6O~B9jiFXFGuocNlhBVar3b2suYqpDH8wmm~4NKvPSMoI4PIuFNXMf9zludZtFiYqr5p9fa3YyFS00Sp~XA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Structure of VB22B sc(Fv)2 minibody. A noncovalent (55 kDa) “diabody” (middle of figure) can be generated by taking the VH and VL regions of a whole anti-Mpl monoclonal IgG (left) and coupling them via a 5-amino acid linker (GGGGS). VB22B sc(Fv)2 is made by binding one VH and one VL fragment together with a 15-amino acid linker [(GGGGS)3] and then binding 2 of these constructs together with the same 15-amino acid linker (middle). Both the diabody and the VB22Bsc(Fv)2 bind and activate the TPO receptor (right). Illustration by Paulette Dennis.
