WT1-specific CD8⁺ T-cell responses in peripheral blood in relation to disease response as measured by WT1/ABL gene expression. Results in 7 individual patients with detectable WT1 gene expression before SCT are shown (A, patient 1; B, patient 2; C, patient 3; D, patient 4; E, patient 5; F, patient 6; G, patient 7). The number of days after transplantation is shown on the x-axis. WT1/HLA-A*0201⁺ CD8⁺ T cells are expressed as absolute numbers/mL of peripheral blood (left, y-axis; dark blue); the shaded area represents absolute numbers of CMVpp65₄₉₅/HLA-A*0201⁺ CD8⁺ T cells. WT1 gene expression in peripheral blood is expressed as the ratio of WT1/ABL (right, y-axis; red). Times of DLI (black arrow), GVHD (med blue), and relapse (green) are depicted on each graph. G indicates GVHD grade; S, skin; P, prednisone; m, months; CyA, cyclosporine A; D, dacluzimab; I, infliximab.