Confocal microscopy of mouse and human platelets and mouse megakaryocytes demonstrate that TFPI does not colocalize with either α granule or lysosomal protein. (A) Human platelets stained for TFPI (green) and fibrinogen as an α granule marker (red). (B) Mouse platelets stained for TFPI (green) and VWF as an α granule marker (red). (C) Mouse megakaryocytes stained for TFPI (green) and VWF as a α granule marker (red). (D) Mouse megakaryocytes stained for TFPI (green) and LAMP1 as a lysosome marker.