Analysis of recombinant mVWF. (A) Multimeric pattern of recombinant murine VWF after hydrodynamic delivery of 100 μg mVWF cDNA. After 24 hours (lanes 2, 5, 7, and 9) or 48 hours (lane 3), plasma was collected and examined for multimeric composition. Lanes 1, 4, 6, and 8 represent normal pooled mouse plasma (NMP). Lanes 2, 3, and 5 represent samples from mice injected with vector mVWF/Cys799, whereas lanes 7 and 9 represent those injected with vector mVWF/Arg799. For comparison, data of 2 laboratories have been included: lanes 1-3 and 6-7 originate from the Institut National de la Santé et de la Recherche Médicale (INSERM) Unité (U) 770 (Le Kremlin-Bicêtre, France), whereas lanes 4-5 and 8-9 originate from the Laboratory for Thrombosis Research (Kortrijk, Belgium). (B) Mice were examined for their bleeding time at 24 hours or 48 hours after hydrodynamic delivery of 100 μg mVWF cDNA using a tail-cut bleeding model. For wild-type mice, bleeding stopped at 93 ± 32 seconds (n = 9). Twenty-four hours after injection with 100 μg mVWF/Cys799-cDNA, the bleeding time was 188 ± 101 seconds. Bleeding did not stop (> 600 seconds) in noninjected mice or mice injected with 100 μg mVWF/Arg799-cDNA.