TF induction is regulated by its cytoplasmic domain in peritoneal macrophages. (A) Multiplex cytokine array analysis of plasma samples from TFΔCT and WT mice treated with an intravenous, single bolus of the Xa inhibitor NAP5 (2 mg/kg) or the thrombin inhibitor hirudin (40 mg/kg) at the time of LPS challenge. Six hours after LPS stimulation, heparinized blood was drawn (n = 8/group). Sham denotes mice that received saline instead of LPS. (B) TAT levels for the treatment groups shown in panel A. (C) TNFα, IL6, IL1β, and MCP1 levels were measured by ELISA in samples obtained 2 hours after LPS challenge. (D) Survival curve of LPS-challenged WT or TFΔCT mice receiving a single intravenous injection of saline or NAP5 (2 mg/kg) at the time of intraperitoneal LPS administration. (E) TF expression by peritoneal macrophages after LPS (5 mg/kg) stimulation. Representative histograms of TF staining of CD11b and F4/80 double-positive peritoneal cells recovered 6 hours after intraperitoneal injection of saline (open histograms) or LPS (shaded histograms). Changes in mean fluorescence over control-treated animals were calculated for each depicted genotype (*denotes different from wild type, P < .05, n ≥ 3).
