Mad2 protein expression in bone marrow cells of Mad2^+/− and Mad2^+/+ mice. Lineage-depleted (lin⁻) and -positive (lin⁺) cells from Mad2^+/+ and Mad2^+/− mice BM were obtained by magnetic separation. Total-cell lysates were dissolved in SDS-PAGE. Membrane was immunoblotted with anti-Mad2 and antiactin antibodies. +/+ indicates Mad2^+/+; +/−, Mad2^+/−.