Figure 7
Analysis of FcγRIIA-induced PLC activation in DAMI cell clones differently expressing LMW-PTP. Intracellular Ca2+ increase was measured using DAMI cells (wild type, S2C, or PTP13 clones, as indicated) loaded with FURA-2-AM, and stimulated by clustering of FcγRIIA with 2 μg/mL IV.3 monoclonal antibody and 30 μg/mL antimouse F(ab′)2 fragments (traces on the left) or with 1 U/mL thrombin (traces on the right). Representative results of at least 3 similar experiments are reported.
