Enhanced AKT activity in CD4⁺CD25⁺ Treg cells does not reduce expression of FOXP3, CTLA-4, CD25, or granzymes A or B. AKT-ER– (or control pCCL-) transduced CD4⁺CD25⁺ Treg cells or CD4⁺CD25⁻ T cells were activated with αCD3/CD28 mAbs in the presence of either vehicle alone (EtOH) or 4HT (150 nM). After 48 hours, cells were stained for CD25, CTLA-4, FOXP3, and granzyme A and B. Unstimulated CD4⁺CD25⁺ and CD4⁺CD25⁻ T-cell lines are included for comparison. A single representative experiment of 4 is depicted.