FLT3 expression in AML cases with trisomy 13 and RUNX1 mutation compared with cases without trisomy 13.FLT3 transcripts were quantified by RQ-PCR on a LightCycler instrument. (A) Unselected AML M0 samples without trisomy 13 (w/o trisomy 13; n = 25) were compared with AML M0 with trisomy 13 plus a RUNX1 mutation (trisomy 13/RUNX1-mutated; n = 8). (B) AML samples from FAB subgroups other than AML M0 with normal karyotype (n = 21) were compared with samples with trisomy 13 and RUNX1 mutation (n = 14). (C) Unselected AML M0 samples together with AML samples from other FAB subgroups without trisomy 13 (w/o trisomy 13; n = 46) were compared with AML with trisomy 13 plus a RUNX1 mutation (trisomy 13/RUNX1-mutated; n = 22). FLT3 expression is given as 100*FLT3/ABL as previously described.21 The bars represent mean values ± SEM. Each sample was analyzed in duplicate.
