Expression of K1 in BJAB cells does not inhibit TRAIL-induced or irradiation-induced apoptosis. (A) BJAB transfectants were treated with 50 ng/mL TRAIL for 4 and 6 hours, fixed, stained with propidium iodide for DNA content, and analyzed by flow cytometry. The number of cells containing subdiploid DNA is indicated by the curve under the horizontal bar at the left of each panel. The percentage of apoptosis in BJABK1, BJABK1m, and BJABXS cells was similar at these intervals. Similar results were obtained in 2 independent experiments. (B) BJAB transfectants were irradiated at 6 or 8 Gy and then incubated for 24 hours, fixed, stained with propidium iodide (PI), and analyzed using flow cytometry. There were no differences in apoptosis rates of BJABK1 cells compared with those of BJABK1m and BJABXS cells. Similar results were obtained in 2 independent experiments.