Effect of ectopically expressed TβRII on the responsiveness of DB cells to TGF-β treatment. (A) DB cells were transfected with either WT or Δcyt of TβRII. After transfection, cells (0.1 × 106 cells/mL) were treated with either medium alone, PMA (0.15 ng/mL), TGF-β (10 ng/mL), or PMA/TGF-β (10 ng/mL) for various periods of time. At the end of each time point, cell counts were performed. (B) An equal amount of nuclear lysates (30 μg) from WT- and Δcyt-transfected DB cells treated with PMA/TGF-β for the time points indicated were analyzed by Western blot analysis. The membranes were probed and reprobed after stripping with different antisera as indicated in (A). (C) An equal amount of nuclear lysates (30 μg) from WT- and Δcyt-transfected DB cells treated with PMA/TGF-β for the time points indicated were analyzed by Western blot analysis. The membranes were probed and reprobed after stripping with different antisera as indicated above.
