Granulysin-mediated anticryptococcal activity did not require perforin to function. (A) Perforin or granulysin steady-state mRNA was assessed by RT-PCR from resting CD4⁺ T cells, activated cells sham electroporated without siRNA (−), and activated cells electroporated with perforin siRNA (P), CD20 siRNA (CD20), or nonspecific siRNA (NS). (B) Granulysin and perforin were detected by Western blot. (C) Activated and treated CD4⁺ T cells were incubated with C neoformans, and the anticryptococcal activity was assessed. Results are expressed as mean ± SEM. *P < .01 compared with the number of C neoformans alone at 24 or 48 hours. Data are representative of 3 independent experiments.