Endogenous TSP1 and NO modulate tissue survival under ischemic conditions. (A) Representative random flaps were photographed 7 days following surgery for untreated WT and TSP1-null mice, WT and TSP1-null mice receiving L-NAME (500 mg/L; B), or mice receiving ISDN (1 mg/mL; C) in the drinking water during the postoperative period. (D) Flap survival is expressed as the percentage of the total involved area. Results are the mean ± SD of 24 animals (12 age- and sex-matched pairs) of untreated WT and TSP1-null mice, 16 animals (8 matched pairs) treated with L-NAME, and 16 animals (8 matched pairs) treated with ISDN. *P < .05 versus control, one-way ANOVA. #P < .05 versus wild type, 2-way ANOVA.