Chemokine and cytokine levels in BAL fluids. Cytokine levels for IL-5, IL-6, TNFα, and MCP-1 were determined from BAL fluids clarified by centrifugation by flow cytometry using the BD mouse Th1/Th2 and BD mouse inflammation cytometric bead array (CBA) kits using the methods outlined by the manufacturers. The levels of mouse KC and MIP-3α were determined by sandwich ELISA according to the manufacturer's instructions. Data are presented as mean ± SD.