Figure 3
Figure 3. Clusterin, CXCL13, PDCD1, NFTAC1, and CD27 immunohistochemistry in AITL and PTCL-u. Representative AITLs (A, C, E, G, and I) and PTCLs-u (B, D, F, H, and J) were immunostained for clusterin (A-B, original magnification ×200, Zeiss Axioscope; Carl Zeiss, Heidelberg, Germany), CXCL13 (C-D, original magnification ×200, Zeiss Axioscope), PDCD1 (E-F, original magnification ×200, Nikon Eclipse 80i; Nikon, Tokyo, Japan), NFATC1 (G-H, original magnification ×400, Zeiss Axioscope), and CD27 (I-J, original magnification ×200, Nikon Eclipse 80i). For panels A-D and G-H, photographs were taken with a DP70 Olympus camera (Tokyo, Japan); images were acquired using DP Controller 2002 (Olympus) and processed using Adobe Photoshop v7.0 (Adobe Systems, San Jose, CA). For panels E-F and I-J, photographs were taken with a CFW-1310C camera (Scion, Frederick, MD); images were acquired using Histolab 5.131.1 (Alphelys, Plaisir, France) and processed using Adobe Photoshop v7.0. Objectives used were Nikon Plan Fluor 20×/0.50 NA; Zeiss Plan Neufluor 20×/0.40 NA; and Zeiss Plan Neofluor 40×/0.60 NA.

Clusterin, CXCL13, PDCD1, NFTAC1, and CD27 immunohistochemistry in AITL and PTCL-u. Representative AITLs (A, C, E, G, and I) and PTCLs-u (B, D, F, H, and J) were immunostained for clusterin (A-B, original magnification ×200, Zeiss Axioscope; Carl Zeiss, Heidelberg, Germany), CXCL13 (C-D, original magnification ×200, Zeiss Axioscope), PDCD1 (E-F, original magnification ×200, Nikon Eclipse 80i; Nikon, Tokyo, Japan), NFATC1 (G-H, original magnification ×400, Zeiss Axioscope), and CD27 (I-J, original magnification ×200, Nikon Eclipse 80i). For panels A-D and G-H, photographs were taken with a DP70 Olympus camera (Tokyo, Japan); images were acquired using DP Controller 2002 (Olympus) and processed using Adobe Photoshop v7.0 (Adobe Systems, San Jose, CA). For panels E-F and I-J, photographs were taken with a CFW-1310C camera (Scion, Frederick, MD); images were acquired using Histolab 5.131.1 (Alphelys, Plaisir, France) and processed using Adobe Photoshop v7.0. Objectives used were Nikon Plan Fluor 20×/0.50 NA; Zeiss Plan Neufluor 20×/0.40 NA; and Zeiss Plan Neofluor 40×/0.60 NA.

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