Induction of antitumor immunity by bortezomib-killed primary myeloma cells without the need for exogenous DC maturation stimulus. Monocyte-derived DCs from patients with MM were pulsed with autologous CD138-positive tumor cells killed by γ irradiation, dexamethasone, or bortezomib in the absence of any DC maturation stimuli and used for the stimulation of autologous T cells for 2 weeks. IFN-γ producers against unpulsed DCs or DCs pulsed with autologous tumor cells were analyzed by an ELISPOT assay. Data shown are representative (mean/SD) of 3 tested patients.