Survivin, an IAP, is up-regulated in trisomy 8. RNA was extracted from purified CD34 cells with trisomy 8 and analyzed for survivin by real-time PCR; FISH was performed on sorted samples. Expression was compared with the mean of a panel of 10 healthy donors, as described in “Materials and methods.” Survivin mRNA was up-regulated in patients with trisomy 8 compared with controls and with a small group of equally cytopenic patients whose cytogenetics showed monosomy 7 (A). Expression was increased to a greater extent in samples with increased trisomy 8 cells (B). Error bars represent SEM.