The inhibition of Prx2 regeneration in erythrocytes treated with DNCB. (A) Erythrocytes were incubated with 30 μM DNCB for the indicated times and immunoblotted. The portions left and right of the dividing line are derived from nonadjacent lanes on the same blot. D indicates dimeric Prx2; M, monomeric Prx2. (B) Erythrocytes were pretreated with CO or 1 mM azide as indicated, then with 30 μM DNCB for 30 minutes. Data are means ± SE from 3 experiments.