The effect of H₂O₂ on the redox state of Prx2 in cells. (A) Erythrocytes were treated at the indicated concentrations for 10 minutes and immunoblotted. Dimeric Prx2 ran as a doublet. (B) Quantification of band intensities as measured by chemiluminescent densitometry. Data are means ± SD of 3 experiments. (C) Erythrocyte suspensions of 5 × 10⁷ cells/mL were treated with the indicated concentrations of H₂O₂ for 10 minutes and immunoblotted. (D) Erythrocytes (5 × 10⁷ cells/mL) were pretreated with 10 mM azide for 5 minutes, then with the indicated concentrations of H₂O₂ for 10 minutes and immunoblotted. (E) Jurkat cells were treated at the indicated concentrations of H₂O₂ for 10 minutes and immunoblotted. Protein (15 μg) was loaded per lane. D indicates dimeric Prx2; M, monomeric Prx2.