The redox state of Prx2 in erythrocytes. (A) Cells were lysed in SDS-sample buffer, analyzed under reducing (R) or nonreducing (NR) conditions, and immunoblotted with antibodies against Prx2. Molecular weight (MW) markers are indicated on the left. (B) Cells were preincubated with or without 100 mM NEM for 15 minutes, then lysed in nonreducing SDS-sample buffer in the absence or presence of 100 mM NEM. Samples were then immunoblotted for the presence of Prx2. D indicates dimeric Prx2; M, monomeric Prx2.