Lmo2 atgMO-mediated knockdown of Lmo2 expression is highly specific. (A-B) Whole-mount 10s embryos; views as indicated; dorsal, top; numbers of embryos represented in gray. (A) Expression of the nonhematopoietic genes nkx2.5 in the precardiac lateral plate mesoderm and pax2.1 in the optic stalk, midbrain/hindbrain boundary region, otic vesicle (arrows, bottom to top), and progenitors of the pronephric duct, immediately adjacent to the primitive erythroid and endothelial precursors in the PLM, was unaffected in morphants. (B) Coinjection of murine lmo2, but not lmo4, mRNA with the lmo2 atgMOs rescued early gata1, pu.1, and flt4 expression levels.