Comparative analysis of IL-11 and IL-11Rα expression and IL-11–induced STAT3 activation in gp130^Y757F/Y757F and gp130^Y757F/Y757F:IL-11Rα1^−/− mice. Q-PCR analysis of (A) IL-11 and IL-11Rα gene expression in thymocytes and (B) IL-11Rα gene expression in whole thymus and liver tissue was performed on cDNA derived from total RNA. Expression data from 3 samples per genotype are shown following normalization for 18S expression and are presented from replicate analysis as the mean fold induction ± SD relative to expression in (A) gp130^+/+ and (B) gp130^Y757F/Y757F samples. (C) Immunoblot analysis of STAT3 tyrosine phosphorylation in liver (Li), lymph node (LN), spleen (Sp), and thymus (Thy) lysates prepared from gp130^Y757F/Y757F (lanes 1, 3, 5, 7) and gp130^Y757F/Y757F:IL-11Rα1^−/− (lanes 2, 4, 6, 8) mice.