Anti-CD95/Fas treatment induces CD95/Fas capping in purified CD8⁺ T cells from HIV⁺ patients but not from healthy donors. (A) Confocal imaging analysis showing CD95/Fas distribution in untreated (left panel) or treated (right panel) Jurkat cells under CD95/Fas-capping conditions. Merged images (blue for DAPI and green for anti-CD95/Fas staining) of representative cells are shown at a single section (top row) or 3D projection (bottom row). (B) CD95/Fas distribution in purified CD8⁺ T cells from an HIV⁺ individual and a healthy donor under treatment with anti-CD95/Fas antibody for 5 minutes or 1 hour at 37°C. White arrows point at CD95/Fas-capping cells. Zoomed images of selected fields are also shown. (C) Representative imaging showing the accumulation of CD95/Fas in uropod-like structures extending from the cell surface. Zoomed images of selected fields are also shown. These results are representative of CD8⁺ T cells from 7 HIV⁺ patients and 4 healthy donors.