Effect of rGel or rGel/BLyS on CLL survival proteins. CLL lymphocytes from 7 patient samples were treated with either 2 and 5 mM rGel or 2 and 5 nM rGel/BLyS for 72 hours, and cell lysates were collected and immunoblot analysis was done for MCL1, BCL2, XIAP, PARP, and actin proteins (A). MCL-1 levels of proteins were quantitated and expressed as percent control from either rGel-treated (B) or rGel/BLyS-treated (C) cells. XIAP levels of proteins were quantitated and expressed as percent control from either rGel-treated (D) or rGel/BLyS-treated (E) cells. Relationship between apoptosis induction measured as PARP cleavage and decrease in MCL-1 (F) or XIAP (G) protein levels after treatment with rGel/BLyS construct.