LPS- but not CD40L-matured LXR agonist–treated DCs fail to up-regulate fascin expression. (A) Immature DCs (iDCs) were differentiated for 7 days and left untreated (control) or treated from day 2 on with 2 μM of the LXR agonist T0901317 (T09). Mature DCs were obtained from iDCs by incubation with LPS or CD40L for the last 2 days. Histograms with open profiles (fine line) represent a staining pattern with an isotype control. T0901317-treated DCs (open profiles with bold line) and untreated DCs (solid profiles) show a staining pattern with the antifascin Ab. Typical histograms and diagrams showing mean of fluorescence intensities related to those obtained from untreated mDCs ± SEM of at least 4 independent experiments are given. Significance versus untreated mDCs: ***P ≤ .001. (B) Typical light microscopy images and immunofluorescence staining of fascin of untreated and T09-treated LPS-matured DCs with different magnifications are shown. Bar represents 10 μm. Similar results were obtained in 4 different experiments.