Effect of LXR agonist treatment on DC morphology, differentiation, and maturation. Immature DCs (iDCs) were differentiated for 7 days and left untreated (control) or treated from day 2 on with 2 μM of the LXR agonists T0901317 (T09) or GW-3965 (GW). Mature DCs were obtained from iDCs by incubation with LPS for the last 2 days. (A) The maturation-induced clustering of DCs and morphologic changes were analyzed by a phase-contrast microscopy. Bar represents 30 μm. Similar results were obtained in 4 different experiments. (B) Surface molecule expression as analyzed by immunofluorescence and flow cytometry. Histograms illustrate staining with isotype control mAb (open profile, fine line), and staining with mAb of the indicated specificity of untreated DCs (solid profiles) and of T0901317-treated (T09) DCs (open profiles, bold line). The logarithm of fluorescence intensities (FIs) is given in the abscissa spanning 4 orders of magnitude. One typical experiment of 10 is shown. Essentially identical results were obtained with GW-3965 (n = 4; data not shown).