Figure 4
![Figure 4. Gfi1b expression in early precursor and hematopoietic stem cells. (A-C) Bone marrow cells from wt or wt/KI (Gfi1b+/GFP) mice, respectively, were depleted for lineage-positive cells (Lin: CD3, CD4, CD8, Mac-1, B220, Ter119, Ly6G, Pan natural killer [NK] plus Sca1, IL7Rα for CMP-GMP-MEP analysis), and negative cells were restained for surface markers as indicated and analyzed by flow cytometry (A-C, left panels). HSCs (A), MEPs, CMPs, GMPs (B), or CLPs (C) were electronically gated as previously described (see “Material and methods”) and analyzed for GFP expression (right panels).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/109/6/10.1182_blood-2006-06-030031/4/zh80060709530004.jpeg?Expires=1725125656&Signature=p2LlTX4SCD-de6pwgZzV~UofoHhJlkFKgiytKLUJ3jPLULkr7afESzcSNo9uH3DzpDIRKFC9MAGkslpe8LXRMjsW~6EiZA9vazE-0k2Q8Yj4GqjWQ-UGF~cJ0Gw8T-jpNiI5CeXp33TLbhzC2kLOgM0x2EQhzPSMbLrhSjF0iXvgqDV3~6984KH5y1uka8zL7ZlfwXtkhs0aqeEed3yZUpwhTCzpTYXZZsMzJbcx1716DaOu6DiFnvZGpDYb-ITaC~gDS1jYF1QB6fHCS1aixrIvk9bWll8-qntq-gCLsRPdOCQqVttYCs773XzKqinEPjSmRHwsP~rsgPolfQp0tA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Gfi1b expression in early precursor and hematopoietic stem cells. (A-C) Bone marrow cells from wt or wt/KI (Gfi1b+/GFP) mice, respectively, were depleted for lineage-positive cells (Lin: CD3, CD4, CD8, Mac-1, B220, Ter119, Ly6G, Pan natural killer [NK] plus Sca1, IL7Rα for CMP-GMP-MEP analysis), and negative cells were restained for surface markers as indicated and analyzed by flow cytometry (A-C, left panels). HSCs (A), MEPs, CMPs, GMPs (B), or CLPs (C) were electronically gated as previously described (see “Material and methods”) and analyzed for GFP expression (right panels).
