Physiologic consequence of CD80 acquisition by memory T cells in vivo. CFSE-labeled DK11.10 memory CD4 T cells were adoptively transferred into either Balb/c or B7DKO mice and rested for 2 more weeks in vivo. Animals were then vaccinated with OVA323-339/adjuvant (alum) and killed 2 days later. CFSE-labeled cells from spleen were analyzed by FACS. Every dot in the graph represents 1 animal. Horizontal bars indicate average. *A statistically significant difference (t test, P < .05). (A) CFSE-labeled cells in B7DKO or Balb/c hosts were analyzed for CD80 acquisition. (B) CFSE-labeled cells in Balb/c hosts were analyzed for CD80 and annexin V. (C) CFSE-labeled cells in B7DKO and Balb/c hosts were analyzed for annexin V. (D) The percentage of CFSE-labeled cells in B7DKO and Balb/c hosts was analyzed.
