Figure 6
Figure 6. Transgenic excess of AChE-R elevates thymic PKC and RACK1 levels. (A) Immunoblot analysis of a single electrophoresis run photographs were cropped as marked by vertical lines. Analysis shows 70- and 60-kDa bands for the FVB/N mouse, a prominent overproduction of a 55-kDa hAChE-R band in TgR mice. TgR thymuses showed PKC (P = .008) and RACK1 overexpression, for equal protein amounts. (B) Hydrolytic AChE activity in TgR. It increased in total thymic homogenates (i) (P < .01), in cytoplasm (ii; P = .003) and in membrane-associated AChE (iii; P = .06) but not in tailed fraction (iv). Error bars show standard error of the mean (SEM). *P < .05.

Transgenic excess of AChE-R elevates thymic PKC and RACK1 levels. (A) Immunoblot analysis of a single electrophoresis run photographs were cropped as marked by vertical lines. Analysis shows 70- and 60-kDa bands for the FVB/N mouse, a prominent overproduction of a 55-kDa hAChE-R band in TgR mice. TgR thymuses showed PKC (P = .008) and RACK1 overexpression, for equal protein amounts. (B) Hydrolytic AChE activity in TgR. It increased in total thymic homogenates (i) (P < .01), in cytoplasm (ii; P = .003) and in membrane-associated AChE (iii; P = .06) but not in tailed fraction (iv). Error bars show standard error of the mean (SEM). *P < .05.

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