Thymopoietic effects of the AChE-R splice variant. (A) Medullary areas in TgR and strain-matched FVB/N controls (P ≤ .01). (B) Heavier thymus weight in TgR mice (P ≤ .009). (C) Total numbers of thymocytes in TgR compared with FVB/N mice (P ≤ .007). (D). Thymuses from TgR mice included fewer CD3^high thymocytes compared with FVB/N controls (P = .065). (E-G) Absolute number of CD4⁺CD8⁺, CD4⁺, and CD8⁺ thymic subsets. Note that, in TgR mice, the increased absolute cell number (panel C) was mainly due to the major double-positive (DP) subset, which was 2-fold increased compared with FVB/N controls (P ≤ .006; panel E), whereas CD4⁺ mature single-positive cells remained unchanged (F) and CD8⁺ subset increased (2-fold) but still remained the minority subset. (H-J) Relative number of those subsets brings further information. Increased CD4⁺CD8⁺ cell percentage (H) in transgenic thymuses (P ≤ .02) counterbalanced by a reduced percentage of CD4⁺ subset (I; P = .075) while the percentage of CD8⁺ subset (J) remained unchanged. Error bars show standard error of the mean (SEM). *P < .05.