PKC and RACK1 expression in MG thymus. (A) Immunolabeled PKC showed increases in cytoplasmic but not membrane-associated PKC labeling from adults to MG patients (P < .001). Most significant increases occurred in the tailed fraction (healthy adults to MG P < .001). (B) Immunoblot analysis of individual thymus samples subjected to a single electrophoresis run. Photographs were cropped as marked by the vertical lines. Cytoplasmic RACK1 showed a mild increase in MG patients compared with healthy adults and no difference in the membrane-associated RACK1. Error bars show standard error of the mean (SEM). *P < .05.