Iron modulates the secretion of s-HJV both in WT and mutants. (A) Transfected HeLa cells were incubated in serum-free media in the presence (+) or absence (−) of 50 μM FAC; after 24 hours, total lysates (50 μg) and concentrated media were analyzed with anti-HJV. Scales refer to relative molecular mass in kilodaltons. (B) Iron modulation of m-HJV was quantified by using the described binding assay. ▪ indicates untreated cells; ⊡, FAC-treated cells. Statistical significance was calculated on 4 independent experiments, made in triplicate. *P < .05. Error bars indicate SD.