PDCs and MDCs in lymph nodes from patients with chronic HIV-1 and patients without HIV-1. Phenotype of cells was characterized by flow cytometry after staining with specific antibodies against surface markers. DCs are defined as viable Lin⁻ HLA-DR⁺ cells expressing particular surface markers CD123 for PDCs and CD11c for MDCs. (A) Flow cytometry of PDCs and MDCs in lymph nodes. Dot-plot of cells lymph nodes from representative patients with chronic HIV-1 (left panel) and patients without HIV-1 (right panel). (B) Number of PDCs and MDCs in lymph nodes in 3 different donors. Presented are values of the cell numbers expressed as percentages of Lin⁻ HLA-DR⁺ cells for sets of 3 tissue blocks derived from each of 3 patients with chronic HIV-1 and 4 patients without HIV-1.