Soluble DC-HIL-Fc enhances elicitation of Ox-induced contact hypersensitivity in mice. Sensitization of BALB/c mice (n = 5) with Ox for CH (A-B): on day 0, mice were painted with 2% Ox on abdominal skin (Senst). On day 6, CH was elicited in sensitized mice by painting 1% Ox or solvent control to right and left ears, respectively (Challenge). CH was assessed daily through day 9 or 12 by measuring ear thickness (▴). Mice were injected intraperitoneally with PBS, hIgG, or DC-HIL-Fc (10 mg/kg each) on days −1, 1, and 3 (before and after sensitization) (A) or on days 5, 7, and 9 (before and after challenge) (B). Daily change in ear thickness was plotted for each panel during sensitization (A) or elicitation (B). *P < .003; **P < .05 compared with ear thickness of mice treated with hIgG. (C) Ear skin was excised from mice treated without (None) or with Ox and Fc protein (2 days after elicitation) and examined histologically (10×/10 objective lens). Data shown (A-B) are representative of 4 independent experiments.