American Society of Hematology

Figure 3

$Figure 3. Absence of B cells in the secondary lymphoid tissues and impaired B-cell development in the bone marrow of Pbx1−/−Rag1−/− chimeric mice. (A) FACS analysis was performed on cells harvested from the spleen, lymph node, and peritoneal cavity of chimeric mice. Staining with anti-B220 (B cells) and anti-Ly9.1 (donor) antibodies showed that all sites in Pbx1−/−Rag1−/− mice were devoid of donor-derived B cells (Ly9.1+B220+). (B) Hardy profile analysis25 was performed on bone marrow cells of chimeric mice. Donor-derived (Ly9.1+) cells from fractions A through C (B220+CD43+) and D through F (B220+CD43−) were completely absent in the bone marrow of the Pbx1−/−Rag1−/− mice. (C) FACS analysis of bone marrow cells for CLPs19 revealed the absence of Ly9.1+ CLPs in Pbx1−/−Rag1−/− chimeras. (D) FACS analysis of bone marrow cells for HSCd revealed the presence of Ly9.1+ HSCs in Pbx1−/−Rag1−/− chimeras.$

Absence of B cells in the secondary lymphoid tissues and impaired B-cell development in the bone marrow of Pbx1^−/−Rag1^−/− chimeric mice. (A) FACS analysis was performed on cells harvested from the spleen, lymph node, and peritoneal cavity of chimeric mice. Staining with anti-B220 (B cells) and anti-Ly9.1 (donor) antibodies showed that all sites in Pbx1^−/−Rag1^−/− mice were devoid of donor-derived B cells (Ly9.1⁺B220⁺). (B) Hardy profile analysis²⁵ was performed on bone marrow cells of chimeric mice. Donor-derived (Ly9.1⁺) cells from fractions A through C (B220⁺CD43⁺) and D through F (B220⁺CD43⁻) were completely absent in the bone marrow of the Pbx1^−/−Rag1^−/− mice. (C) FACS analysis of bone marrow cells for CLPs¹⁹ revealed the absence of Ly9.1⁺ CLPs in Pbx1^−/−Rag1^−/− chimeras. (D) FACS analysis of bone marrow cells for HSCd revealed the presence of Ly9.1⁺ HSCs in Pbx1^−/−Rag1^−/− chimeras.

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