Increased immunogenicity of Bim^−/− DCs in vivo. (A) CFSE-labeled OT1 or OT2 T cells were injected into recipient mice. DCs from WT or Bim^−/− mice with or without LPS stimulation were pulsed with corresponding OVA peptides, or PBS alone, and then injected into recipient mice. Three days later, CFSE⁺ cells in the draining nodes were analyzed for CFSE dilution of dividing cells. The average numbers of cell division (means ± SEM) from 5 independent mice were also calculated. Statistical significance was analyzed by Student t test, and the P values are as follows: for mice injected with OT2 cells, .001 (WT vs Bim^−/−) and .001 (WT + LPS vs Bim^−/− + LPS); and for mice injected with OT1 cells, .001 (WT vs Bim^−/−) and .001 (WT + LPS vs Bim^−/− + LPS). (B) CD8⁺ OVA-specific T cells in the draining lymph nodes in panel A were also detected by staining with anti-CD8 and H-2k^b/OVA tetramer. The percentages of CD8⁺K^b/OVA⁺ cells are averages of 5 independent mice (means ± SEM). Statistical significance was analyzed by Student t test, and the P values are as follows: .001 (WT vs Bim^−/−), .001 (WT + LPS vs Bim^−/− + LPS).