Bim^−/− DCs in T-cell stimulation. (A) CD4⁺ OT2 cells (10⁵/well) were cultured with indicated numbers of wild-type (WT) or Bim^−/− CD11c⁺ DCs with or without pulsing of OVA_323-229 peptide. Three days later, the cells were labeled with [³H]-thymidine during the last 8 hours of culture to measure [³H]-thymidine incorporation. Data (mean ± SEM) are averages of 3 independent experiments. (B) CD8⁺ OT1 cells (10⁵/well) were cultured with indicated numbers of WT or Bim^−/− CD11c⁺ DCs with or without pulsing of OVA_SIINFEKL peptide. [³H]-thymidine incorporation was measured 3 days later. Data (mean ± SEM) are averages of 3 independent experiments. (C) CD11c⁺ BMDCs from WT or Bim^−/− mice were treated with various TLR stimuli as indicated. IL-12p70 in the supernatants was quantitated by ELISA. Data (mean ± SD) are representative of 3 experiments. (D) CD11c⁺ BMDCs from WT or Bim^−/− mice were treated with various TLR stimuli and were used for intracellular staining for IL-12 by flow cytometry. The percentage of IL-12–producing cells and the mean fluorescence staining (MFI) (means ± SD) for IL-12 staining were plotted.