Roles of Bim in spontaneous apoptosis in DCs with or without TLR stimulation. (A) CD11c⁺ BMDCs were cultured in the presence or absence of GM-CSF for 24 hours. Cells were either lysed for Western blot analyses (left) or used to measure cell loss (right). (B) CD11c⁺ BMDCs derived from WT or Bim^−/− mice were used for Western blot analyses (left). Spontaneous apoptosis of WT or Bim^−/− CD11c⁺ BMDCs with or without treatments with different TLR stimuli were quantitated 24 hours later (right). Data shown (mean ± SEM) are averages of 3 independent experiments.