Immunocytochemistry and Western blotting for granule proteins. (A-J) Immunocytochemistry for neutrophil granule proteins; bar, 20 μm; arrows point toward promyelocytes and arrowheads toward myelocytes. (A,C,E,G,I) Bone marrow cells from wild-type mice. (B,D,F,H,J) Bone marrow cells from serglycin knockout mice. (A-B) MPO and (C-D) BPI protein, azurophil granule proteins, (E-F) 24p3 and (G-H) lactoferrin (specific granule proteins), and (I-J) gelatinase B (MMP-9) (gelatinase granule protein). (K-N) Western blotting for 24p3 (K), lactoferrin (L), MMP-9 (M), and MPO (N), lysates of bone marrow cells from wild-type (+/+) and serglycin knockout mice (−/−); molecular weight markers are indicated.