Expression pattern of Gfi-1, PU.1, C/EBPϵ, and their downstream targets in ATRA-induced Gfi-1^+/+ and Gfi-1^+/– EPRO cells. (A) Real-time PCR analysis of ATRA-induced Gfi-1^+/+ and Gfi-1^+/− EPRO cells. EPRO cells were induced to terminally differentiate in the presence of ATRA. RNA samples were collected at the times indicated and subjected to real-time PCR analysis. Transcript levels of each mRNA were normalized to that of 18S rRNA and expressed as a percentage of the signal observed in the uninduced EPRO^+/+ cells. This experiment was performed 3 times in triplicate. (B) Western blot analysis of ATRA-induced Gfi-1^+/+ and Gfi-1^+/− EPRO cells. Whole cell extracts prepared from ATRA-induced EPRO cells at the time indicated were subjected to Western blot analysis. Equal concentrations of protein were loaded in each lane. The blots were sequentially probed with antibodies for LF and β-actin, and NC and β-actin.