Expression of PU.1 and Gfi-1 in the SGD patient. Real-time PCR analysis of (A) PU.1 and (B) Gfi-1 expression in normal versus SGD PMNs. Transcript levels of each mRNA were assessed in triplicate and normalized to that of β-actin and expressed as a percentage of the signal observed in the normal sample (100%). Due to the paucity of RNA, this experiment was performed only once in triplicate. Error bars indicate SEM. (C) Western blot analysis of PMNs from SGD and normal samples. Nuclear extracts prepared from peripheral blood neutrophils (PMNs) of 2 healthy volunteers and our SGD patient were subjected to Western blot analysis. Equal concentrations of protein were loaded in each lane. The blot was sequentially probed with antibodies for Gfi-1, PU.1, and β-actin.