Transient cotransfection analysis of SGP promoter plasmids (HNC and LF) with wild-type and mutant C/EBPϵ expression plasmids in 32Dwt18 cells. 32Dwt18 cells were transiently cotransfected with HNC193 and LF477 (SGP) reporter plasmids (see “Patients, materials, and methods”) with 10 μg expression plasmids for wt C/EBPϵ (e) and mutant C/EBPϵ (V218A) either separately or together. Normalized luciferase values have been represented as a ratio of enzyme activity of SGP promoter plasmids plus C/EBPϵ expression plasmids to that of the SGP promoter plasmid without expression plasmids. The figure represents normalized mean ± SE obtained from 3 independent experiments, each performed in duplicate. Statistical analysis performed by Student t test revealed no statistical significance in the transactivation ability of wt and mutant plasmids. *P = .15; †P = .22.